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KMID : 0603820130190020118
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Journal of Experimental & Biomedical Science
2013 Volume.19 No. 2 p.118 ~ p.123
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Differential Effects of TNF-α on the Survival and Apoptosis of Human Granulocytes and the Human Myeloid Leukemia Cell Line
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Yang Eun-Ju
Chang Jeong-Hyun
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Abstract
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Tumor necrosis factor-alpha (TNF-α) is a proinflammatory cytokine that mediates the inflammatory response and immune functions, and modulates the proliferation, differentiation and cell death of cancer cells. The differential functions of TNF-α in various human cells due to the formation of different stimulating pathway upon the binding of TNF-α to its receptors. In the present study, we examined the different effects of TNF-α on the survival and apoptosis between normal granulocytes and human myeloid leukemia HL-60 cells. Although TNF-α did not affect on the constitutive apoptosis of granulocytes, TNF-α strongly induced the apoptosis of HL-60 cells in a dose- and a time-dependent manner. TNF-α-induced apoptosis was occurred via the activation of caspase 8, caspase 9 and caspase 3/7 and the induction of ROS production in HL-60 cells. Also, BAY-11-7085, a NF-κB inhibitor, blocked the TNF-α-induced apoptosis in HL-60 cells. NF-κB may be involved in TNF-α-induced apoptotic signaling pathway in HL-60 cells. These results suggest that TNF-α activates apoptotic pathways and its process depends on cell type and many cellular factors. A better understanding of the differential effect of TNF-α on cell apoptosis and survival may provide important information that can be used to elucidate the specific inhibitory effect of TNF-α on the cancer dis.
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KEYWORD
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Apoptosis, Granulocyte, HL-60 cells, Survival, TNF-α
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